University of Tehran

 Leptospira Research Laboratory


Postal address: Dr  G. Abdollahpour,  P. O.  Box: 14155-6453  Tehran,  Iran.

Fax:  0098  26  36204023   and  0098  21 66933222


Director:  G. ABDOLLAHPOUR, PhD,  WHO/LERG advisor

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Leptospirosis is an infectious disease caused by pathogenic bacteria called leptospira that can be transmitted directly or indirectly from animals to humans. It is therefore a zoonosis. It's clinical manifestations are so variable that infection can not be detected or proved reliably only on the basis of symptoms. Therefore, diagnosis can only be established by demonstration of the etiological agent or by serological procedures. In Iran, the earliest recognised report of leptospirosis is published by Rafyi and Magami in 1968. 



Leptospira research laboratory has been officially opened in 1999, by funding of Tehran University and the help of WHO/FAO Collaborating Center for Reference & Research on Leptospirosis, and Professor W. A. Ellis. Since then many research projects covering different aspects of Leptospirosis have been carried out in this Laboratory.


The main prospect of this laboratory is to investigate the current situation of leptospirosis in Iran and provide materials such as specific culture media (GRA-SINA media), standard antiserum against reference strain of Leptospira, diagnostic kits and technical support for Iranian researchers to pursue their research in this field. Also we can provide these materials and to researchers in other countries. We also offering to help and provide consultation and training on diagnostic methods and establishment of Leptospira Research Center in other countries or Universities.

At the present two postgraduates students and two graduate students are conducting their thesis research projects in this Laboratory.


Current research projects conducted in this Laboratory:


1) Identification of leptospira isolated from animals in Iran using REA technique.

2) Comparison of three techniques to detect  leptospira interrogans in urine samples.

3) Sero-epidemiological study of human and bovine leptospirosis in North of Iran  (DVM thesis  project).

4) Sero-Bacteriolocal survey of Leptospirosis in infected dairy herd in Tehran suburbs (PhD project)

5) Characterization of leptospires cause acute human leptospirosis isolated from patient referred to hospitals of Gilan Medical Science University by PCR

 6) Study of  leptospirosis in shepherd dogs Tehran Province of Iran  (DVM thesis  project).

 7) Sero-epidemiological study of leptospirosis in camel in Qum Province of Iran  (DVM thesis  project).

 8) Study of leptospirosis in slaughterhouse's employees in Kurdistan Province of Iran  (Research  project).

 9) Study of leptospirosis in suspected cases in Mazandaran Province of Iran  (Research  project).

 10) Seroepidemiological study of Leptospirosis in dairy farms in Sabzevar area in Iran  (DVM  project).

12) Seroprevalence of leptospirosis in sheep and farmers of Khoey city of Azerbayjan (DVM thesis project)

13) A survey on Leptospiral,Campylobacterial and Brucellal abortion frequency rates in the samples collected from the dairy herds in suburb of Tabriz by PCR and serological methods (Research project)

14) Sero-epidemiological study of feline leptospirosis in Mashhad. (Research project)

15) The role of Leptospirosis in abortion in an industrial dairy farm in Isfahan. (Research Project)

16) The influence of leptospira serotypes on occurrence of clinical features of leptospirosis in cows using MAT, culture and PCR in Tehran suburbs dairy farms

17) Serological survey of Leptospirosis in some patients with kidney failure in Tehran (Research Project)

18) Study of Leptospirosis in Kurdistan province of Iran (DVM Research Project)

19) Serological and etiological investigation (culture and PCR) of Leptospirosis among ranchers and cattles in Markazi province

20) Sero-epidemiological investigation and molecular typing of isolated pathogenic leptospires from livestock in Guilan province

21) Serological and molecular investigation of Leptospirosis in feral cats in Mashhad district using MAT and PCR

22) Seroepidemiologic study of leptospirosis in slaughter house personnel of Gilan Province using MAT technique and verification of the positive samples using Nested PCR





The different diagnostic tests which are used in this laboratory include:


1.  Direct examination of the infected materials (more details).




2.   Microscopic Agglutination Test (MAT) on serum samples (more details)






3.   Culture of the Leptospira from urine, blood or milk (more details)





4.   Fluorescent Antibody Test (more details)





5.   ELISA on serum samples (more details)





6.   Polymerase chain reaction  (more details)








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